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Journal: npj Imaging
Article Title: Simultaneous three-photon and optical coherence microscopy deep within an intact mouse brain
doi: 10.1038/s44303-026-00148-9
Figure Lengend Snippet: The multimodal imaging system includes two paths, the OCM path and the THG/3P fluorescence path. The three modalities, OCM/ THG/3P, share the same laser source, the scanning unit and the imaging optics. The THG/3P path employed two PMTs (PMT1 photomultiplier tube to measure 3P fluorescence, PMT2 photomultiplier tube to measure THG), two dichroic mirrors (DM2 dichroic mirror to separate 3P excitation beam from emission beam, DM1 dichroic mirror to separate THG and 3P signal), and bandpass filters on each path to further select the signals. The scanning unit and signal collection system (two PMTs for THG and 3P, InGaAs line CCD-based high-speed spectrometer for OCM) are synchronized for signal collection under computer control. CPA: Chirped Pulse Amplification (Coherent Inc.). OPA optical parametric amplifier (Opera-F, Coherent Inc.). BS beam splitter. PBS polarizing beam splitter. PMT photomultiplier tubes. Lens focal length: 150 mm for L1–L3, 750 mm for L4, and 6.24 mm for L5. The input port of single-mode fiber (SMF) of the fiber-coupled spectrometer acts as the confocal “pinhole”, which is optically conjugate to the objective’s focal plane.
Article Snippet: For the SD-OCM setup, the backscattered light was de-scanned, refocused by an aspherical lens (A110TM-C, Thorlabs) into the
Techniques: Imaging, Fluorescence, Control, Amplification